AUTHOR=Dong Huixia , Zhang Wei , Li Yongxia , Feng Yuqian , Wang Xuan , Liu Zhenkai , Li Dongzhen , Wen Xiaojian , Ma Shuai , Zhang Xingyao 

TITLE=Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.973305

DOI=10.3389/fpls.2022.973305

ISSN=1664-462X

ABSTRACT=<p>Salicylic acid (SA) is generally considered to be a critical signal transduction factor in plant defenses against pathogens. It could be converted to methyl salicylate (MeSA) for remote signals by salicylic acid methyltransferase (SAMT) and converted back to SA by SA-binding protein 2 (SABP2). In order to verify the function of SAMT in poplar plants, we isolated the full-length cDNA sequence of <italic>PagSAMT</italic> from 84K poplar and cultivated <italic>PagSAMT</italic> overexpression lines (OE-2 isolate) to test its role in SA-mediated defenses against the virulent fungal pathogen <italic>Botryosphaeria dothidea</italic>. Our results showed that after inoculation with <italic>B. dothidea</italic>, OE-2 significantly increased MeSA content and reduced SA content which is associated with increased expression of <italic>SAMT</italic> in both infected and uninfected leaves, when compared against the wild type (WT). Additionally, <italic>SAMT</italic> overexpression plant lines (OE-2) exhibited higher expression of pathogenesis-related genes <italic>PR-1</italic> and <italic>PR</italic>-5, but were still susceptible to <italic>B. dothidea</italic> suggesting that in poplar SA might be responsible for resistance against this pathogen. This study expands the current understanding of joint regulation of SAMT and SABP2 and the balance between SA and MeSA in poplar responses to pathogen invasion.</p>