AUTHOR=Muñoz-Avila Julio C. , Prieto Concepción , Sánchez-Sevilla José F. , Amaya Iraida , Castillejo Cristina 

TITLE=Role of FaSOC1 and FaCO in the seasonal control of reproductive and vegetative development in the perennial crop Fragaria × ananassa

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.971846

DOI=10.3389/fpls.2022.971846

ISSN=1664-462X

ABSTRACT=<p>The diploid woodland strawberry (<italic>F. vesca</italic>) represents an important model for the genus <italic>Fragaria</italic>. Significant advances in the understanding of the molecular mechanisms regulating seasonal alternance of flower induction and vegetative reproduction has been made in this species. However, this research area has received little attention on the cultivated octoploid strawberry (<italic>F.</italic> × <italic>ananassa</italic>) despite its enormous agronomical and economic importance. To advance in the characterization of this intricated molecular network, expression analysis of key flowering time genes was performed both in short and long days and in cultivars with seasonal and perpetual flowering. Analysis of overexpression of <italic>FaCO</italic> and <italic>FaSOC1</italic> in the seasonal flowering ‘Camarosa’ allowed functional validation of a number of responses already observed in <italic>F. vesca</italic> while uncovered differences related to the regulation of FaFTs expression and gibberellins (GAs) biosynthesis. While FvCO has been shown to promote flowering and inhibit runner development in the perpetual flowering H4 accession of <italic>F. vesca</italic>, our study showed that <italic>FaCO</italic> responds to LD photoperiods as in <italic>F. vesca</italic> but delayed flowering to some extent, possibly by induction of the strong <italic>FaTFL1</italic> repressor in crowns. A contrasting effect on runnering was observed in FaCO transgenic plants, some lines showing reduced runner number whereas in others runnering was slightly accelerated. We demonstrate that the role of the MADS-box transcription factor FaSOC1 as a strong repressor of flowering and promoter of vegetative growth is conserved in woodland and cultivated strawberry. Our study further indicates an important role of FaSOC1 in the photoperiodic repression of FLOWERING LOCUS T (FT) genes <italic>FaFT2</italic> and <italic>FaFT3</italic> while <italic>FaTFL1</italic> upregulation was less prominent than that observed in <italic>F. vesca</italic>. In our experimental conditions, FaSOC1 promotion of vegetative growth do not require induction of GA biosynthesis, despite GA biosynthesis genes showed a marked photoperiodic upregulation in response to long days, supporting GA requirement for the promotion of vegetative growth. Our results also provided insights into additional factors, such as FaTEM, associated with the vegetative developmental phase that deserve further characterization in the future.</p>