AUTHOR=Yin Meng , Wang Rui , Li Shi , Luo Mei , Wei Wei , Wang Maolin , Jiang Jun , Lin Yongjun , Zhao Yun 

TITLE=High Sclerotinia sclerotiorum resistance in rapeseed plant has been achieved by OsPGIP6

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.970716

DOI=10.3389/fpls.2022.970716

ISSN=1664-462X

ABSTRACT=<p><italic>Sclerotinia sclerotiorum</italic>, a worldwide distributed fungal pathogen, causes serious adverse effects on the yield and seed quality of rapeseed. Polygalacturonase-inhibiting proteins (PGIPs) can protect the cell wall from degradation by pathogen-secreted polygalacturonases (PGs). The present study found several PGIPs from <italic>Oryza sativa</italic>, especially OsPGIP6 and 3 have much higher inhibitory activities to SsPGs than BnPGIP2 from <italic>Brassica napus</italic>. Among them, <italic>OsPGIP1, 4, 6</italic> can significantly elevate the resistance of transgenic Arabidopsis to <italic>S. sclerotiorum</italic>. Subsequently, <italic>OsPGIP1, 3, 4, 6</italic> were subjected to SSR resistance assay in transgenic rapeseed plants. Among which, <italic>OsPGIP6</italic> showed the highest resistance to <italic>S. sclerotiorum</italic>. At 48 h after detached leaves inoculation, the lesion area of OE-OsPGIP6 rapeseed plants is only 17.93% of the non-transgenic line, and 22.17, 21.32, 52.78, 56.47%, compared to OE-BnPGIP2, OE-OsPGIP1, OE-OsPGIP2, OE-OsPGIP4, respectively. Furthermore, the lesion area of OE-OsPGIP6 reached 10.11% compared to WT at 72 hpi. Also, the lesion length on the stem of OE-OsPGIP6 plants was reduced by 36.83% compared to WT. These results reveal that <italic>OsPGIP</italic> family, especially <italic>OsPGIP6</italic>, has a great potential in rapeseed <italic>S. sclerotiorum</italic>-resistance breeding.</p>