AUTHOR=Shim Kyu-Chan , Adeva Cheryl , Kang Ju-Won , Luong Ngoc Ha , Lee Hyun-Sook , Cho Jun-Hyeon , Kim HyunJung , Tai Thomas H. , Ahn Sang-Nag 

TITLE=Interaction of starch branching enzyme 3 and granule-bound starch synthase 1 alleles increases amylose content and alters physico-chemical properties in japonica rice (Oryza sativa L.)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.968795

DOI=10.3389/fpls.2022.968795

ISSN=1664-462X

ABSTRACT=<p>Four near-isogenic lines (NILs) with different allele combinations of the <italic>starch branching enzyme 3</italic> (<italic>SBE3</italic>) and <italic>granule-bound starch synthase 1</italic> (<italic>GBSS1</italic>) were developed by crossing the <italic>japonica</italic> rice cultivars “Dodamssal” and “Hwayeong.” The associations between sequence variations in <italic>SBE3</italic> and <italic>GBSS1</italic>, and starch-related traits were investigated. These sequence variations led to changes in seed morphology, starch structure, starch crystallinity, amylopectin chain length distribution, digestibility, apparent amylose content (AAC), and resistant starch content (RS). <italic>SBE3</italic> and <italic>GBSS1</italic> showed genetic interaction in regulating AAC and RS. Gene expression profiling of panicle tissues revealed significant differences in expression levels of <italic>GBSS1</italic>, <italic>SBE3</italic>, and other starch-related genes among the four NILs, indicating that variations in <italic>GBSS1</italic> and <italic>SBE3</italic> changed the expression level of starch-related genes. These variations contributed to the changes observed in AAC, RS, and physico-chemical characteristics of the rice starch from the NILs.</p>