AUTHOR=Zhao Jianping , Jiang Lihui , Bai Hanrui , Dai Yuliang , Li Kuixiu , Li Saijie , Wang Xiaoran , Wu Lixia , Fu Qijing , Yang Yanfen , Dong Qian , Yu Si , Wang Meixian , Liu Haiyan , Peng Ziai , Zhu Haiyan , Zhang Xiaoyan , He Xie , Lei Yan , Liang Yan , Guo Liwei , Zhang Hongji , Yu Decai , Liu Yixiang , Huang Huichuan , Liu Changning , Peng Sheng , Du Yunlong 

TITLE=Characteristics of members of IGT family genes in controlling rice root system architecture and tiller development

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.961658

DOI=10.3389/fpls.2022.961658

ISSN=1664-462X

ABSTRACT=<p>Root system architecture (RSA) and tiller are important agronomic traits. However, the mechanisms of the IGT family genes regulate RSA and tiller development in different rice varieties remain unclear. In this study, we demonstrated that 38 rice varieties obtained from Yuanyang Hani’s terraced fields with different RSA and could be classified into six groups based on the ratio of root length and width. We found a positive correlation between RSA (including root width, length, and area) and tiller number in most of rice varieties. Furthermore, the IGT family genes <italic>Deeper Rooting 1</italic> (<italic>DRO1</italic>), <italic>LAZY1</italic>, <italic>TAC1</italic>, and <italic>qSOR1</italic> showed different expression patterns when rice grown under irrigation and drought conditions. Moreover, the <italic>qSOR1</italic> gene had higher levels in the roots and tillers, and accompanied with higher levels of <italic>PIN1b</italic> gene in roots when rice grown under drought environmental condition. <italic>DRO1</italic> gene had two single nucleotide polymorphisms (SNPs) in the exon 3 sequences and showed different expression patterns in the roots and tillers of the 38 rice varieties. Overexpression of <italic>DRO1</italic> with a deletion of exon 5 caused shorter root length, less lateral roots and lower levels of <italic>LAZY1</italic>, <italic>TAC1</italic>, and <italic>qSOR1</italic>. Further protein interaction network, microRNA targeting and co-expression analysis showed that <italic>DRO1</italic> plays a critical role in the root and tiller development associated with auxin transport. These data suggest that the RSA and tiller development are regulated by the IGT family genes in an intricate network way, which is tightly related to rice genetic background in rice adapting to different environmental conditions.</p>