AUTHOR=Gandass Nishu , Kajal  , Salvi Prafull 

TITLE=Intrinsically disordered protein, DNA binding with one finger transcription factor (OsDOF27) implicates thermotolerance in yeast and rice

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.956299

DOI=10.3389/fpls.2022.956299

ISSN=1664-462X

ABSTRACT=<p>Intrinsically disorder regions or proteins (IDRs or IDPs) constitute a large subset of the eukaryotic proteome, which challenges the protein structure–function paradigm. These IDPs lack a stable tertiary structure, yet they play a crucial role in the diverse biological process of plants. This study represents the intrinsically disordered nature of a plant-specific DNA binding with one finger transcription factor (DOF-TF). Here, we have investigated the role of <italic>OsDOF27</italic> and characterized it as an intrinsically disordered protein. Furthermore, the molecular role of <italic>OsDOF27</italic> in thermal stress tolerance has been elucidated. The qRT-PCR analysis revealed that <italic>OsDOF27</italic> was significantly upregulated under different abiotic stress treatments in rice, particularly under heat stress. The stress-responsive transcript induction of <italic>OsDOF27</italic> was further correlated with enriched abiotic stress-related <italic>cis</italic>-regulatory elements present in its promoter region. The <italic>in vivo</italic> functional analysis of the potential role of <italic>OsDOF27</italic> in thermotolerance was further studied in yeast and <italic>in planta</italic>. Ectopic expression of <italic>OsDOF27</italic> in yeast implicates thermotolerance response. Furthermore, the rice transgenic lines with overexpressing <italic>OsDOF27</italic> revealed a positive role in mitigating heat stress tolerance. Collectively, our results evidently show the intrinsically disorderedness in <italic>OsDOF27</italic> and its role in thermal stress response in rice.</p>