AUTHOR=Kamdar Jignesh H. , Jasani Mital D. , Chandrashekar Ajay B. , Janila Pasupulati , Pandey Manish K. , Georrge John J. , Varshney Rajeev K. , Bera Sandip K. 

TITLE=Does improved oleic acid content due to marker-assisted introgression of ahFAD2 mutant alleles in peanuts alter its mineral and vitamin composition?

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.942617

DOI=10.3389/fpls.2022.942617

ISSN=1664-462X

ABSTRACT=<p>Peanuts (<italic>Arachis hypogaea</italic> L.) with high oleic acid content have extended shelf life and several health benefits. Oleic, linoleic, and palmitic acid contents in peanuts are regulated by <italic>ahFAD2A</italic> and <italic>ahFAD2B</italic> mutant alleles. In the present study, <italic>ahFAD2A</italic> and <italic>ahFAD2B</italic> mutant alleles from SunOleic 95R were introgressed into two popular peanut cultivars, GG-7 and TKG19A, followed by markers-assisted selection (MAS) and backcrossing (MABC). A total of 22 MAS and three MABC derived lines were developed with increased oleic acid (78–80%) compared to those of GG 7 (40%) and TKG 19A (50%). Peanut kernel mineral and vitamin composition remained unchanged, while potassium content was altered in high oleic ingression lines. Two introgression lines, HOMS Nos. 37 and 113 had over 10% higher pooled pod yield than respective best check varieties. More than 70% recurrent parent genome recovery was observed in HOMS-37 and HOMS-113 through recombination breeding. However, the absence of recombination in the vicinity of the target locus resulted in its precise introgression along with ample background genome recovery. Selected introgression lines could be released for commercial cultivation based on potential pod yield and oleic acid content.</p>