AUTHOR=Yu Qing , Wang Haijun , Wang Hongzhu , Xu Chao , Liu Miao , Ma Yu , Li Yan , Ma Shuonan , Hamilton David P. , Jeppesen Erik 

TITLE=Effects of High Ammonium Loading on Two Submersed Macrophytes of Different Growth Form Based on an 18-Month Pond Experiment

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.939589

DOI=10.3389/fpls.2022.939589

ISSN=1664-462X

ABSTRACT=<p>Ammonium (NH<sub>4</sub>-N) produces a paradoxical effect on submersed macrophytes because it is not only the preferred nitrogen source for the growth of plants but also threatens the growth of plants at high concentration. Whether short-term and small-scale physiological toxicity experiments at an individual level can reflect the effects of high ammonium on populations of submersed macrophytes in natural conditions is still unclear. In this study, an 18-month experiment was conducted in six 600 m<sup>2</sup> ponds subjected to different levels of ammonium loading. The effects of high ammonium on populations of canopy-forming <italic>Myriophyllum spicatum</italic> and rosette-forming <italic>Vallisneria natans</italic> were explored. The results showed that <italic>M. spicatum</italic> and <italic>V. natans</italic> populations can develop high cover and height at high ammonium concentration (7 mg/L) at short-term exposures, and <italic>V. natans</italic> may be tolerant to 18 mg/L ammonium concentration. However, the cover of <italic>M. spicatum</italic> and the height of both species were inhibited at 2.4 mg/L at long-term exposures. The height of <italic>M. spicatum</italic> was two to six times higher than that of <italic>V. natans</italic> across all treatments and control by the end of the experiment, and the cover of <italic>M. spicatum</italic> was 7–11 times higher than that of <italic>V. natans</italic> in most NH<sub>4</sub>-N loading treatments, except the cover of <italic>M. spicatum</italic> in the highest NH<sub>4</sub>-N loading treatment with 18 mg/L NH<sub>4</sub>-N. The rosette-forming <italic>V. natans</italic> resists ammonium stress by slow growth (shoot elongation) to reduce consumption, while canopy-forming species resist ammonium stress by shoot elongation and canopy development to capture light. Although increasing ammonium concentration may induce severe stress on <italic>M. spicatum</italic>, the morphological characteristics of this species may, to some extent, release the plants from this stress. Our present study indicates that the negative effects of ammonium stress on the development of populations increased with exposure duration, and the submersed macrophyte community with stronger ability for light capture and dispersal may resist high ammonium stress. Nevertheless, in strongly ammonium-enriched systems, competition and succession cannot be neglected.</p>