AUTHOR=Song Linhu , Wang Xingmei , Zou Liangping , Prodhan Zakaria , Yang Jiaheng , Yang Jianping , Ji Li , Li Guanhui , Zhang Runcong , Wang Changyu , Li Shi , Zhang Yan , Ji Xiang , Zheng Xu , Li Wanchen , Zhang Zhiyong 

TITLE=Cassava (Manihot esculenta) Slow Anion Channel (MeSLAH4) Gene Overexpression Enhances Nitrogen Assimilation, Growth, and Yield in Rice

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.932947

DOI=10.3389/fpls.2022.932947

ISSN=1664-462X

ABSTRACT=<p>Nitrogen is one of the most important nutrient elements required for plant growth and development, which is also immensely related to the efficient use of nitrogen by crop plants. Therefore, plants evolved sophisticated mechanisms and anion channels to extract inorganic nitrogen (nitrate) from the soil or nutrient solutions, assimilate, and recycle the organic nitrogen. Hence, developing crop plants with a greater capability of using nitrogen efficiently is the fundamental research objective for attaining better agricultural productivity and environmental sustainability. In this context, an in-depth investigation has been conducted into the cassava slow type anion channels (<italic>SLAHs</italic>) gene family, including genome-wide expression analysis, phylogenetic relationships with other related organisms, chromosome localization, and functional analysis. A potential and nitrogen-responsive gene of cassava (<italic>MeSLAH4</italic>) was identified and selected for overexpression (OE) analysis in rice, which increased the grain yield and root growth related performance. The morpho-physiological response of OE lines was better under low nitrogen (0.01 mm NH<sub>4</sub>NO<sub>3</sub>) conditions compared to the wild type (WT) and OE lines under normal nitrogen (0.5 mm NH<sub>4</sub>NO<sub>3</sub>) conditions. The relative expression of the <italic>MeSLAH4</italic> gene was higher (about 80-fold) in the OE line than in the wild type. The accumulation and flux assay showed higher accumulation of <inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mrow><mml:mi mathvariant="normal">NO</mml:mi></mml:mrow><mml:mn>3</mml:mn><mml:mo>−</mml:mo></mml:msubsup></mml:mrow></mml:math></inline-formula> and more expansion of root cells and grain dimension of OE lines compared to the wild type plants. The results of this experiment demonstrated that the <italic>MeSLAH4</italic> gene may play a vital role in enhancing the efficient use of nitrogen in rice, which could be utilized for high-yielding crop production.</p>