AUTHOR=Zhou Xinli , Li Xin , Han Dejun , Yang Suizhuang , Kang Zhensheng , Ren Runsheng 

TITLE=Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.932762

DOI=10.3389/fpls.2022.932762

ISSN=1664-462X

ABSTRACT=<p>Winter wheat cultivar Pindong 34 has both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust, which is caused by <italic>Puccinia striiformis</italic> f. sp. <italic>tritici</italic> (<italic>Pst</italic>). To map the quantitative trait loci (QTL) for stripe rust resistance, an F<sub>6−10</sub> recombinant inbred line (RIL) population from a cross of Mingxian 169 × Pingdong 34 was phenotyped for stripe rust response over multiple years in fields under natural infection conditions and with selected <italic>Pst</italic> races under controlled greenhouse conditions, and genotyping was performed with a 90K single nucleotide polymorphism (SNP) array chip. Inclusive composite interval mapping (ICIM) identified 12 APR resistance QTLs and 3 ASR resistance QTLs. Among the 12 APR resistance QTLs, <italic>QYrpd.swust-1BL</italic> (explaining 9.24–13.33% of the phenotypic variation), <italic>QYrpd.swust-3AL.1</italic> (11.41–14.80%), <italic>QYrpd.swust</italic>-<italic>3AL.2</italic> (11.55–16.10%), <italic>QYrpd.swust-6BL</italic> (9.39–12.78%), <italic>QYrpd.swust-6DL</italic> (9.52–16.36%), <italic>QYrpd.swust-7AL</italic> (9.09–17.0%), and <italic>QYrpd.swust-7DL</italic> (8.87–11.38%) were more abundant than in the five tested environments and <italic>QYrpd.swust-1AS</italic> (11.05–12.72%), <italic>QYrpd.swust-1DL</italic> (9.81–13.05%), <italic>QYrpd.swust-2BL.1</italic> (9.69–10.57%), <italic>QYrpd.swust-2BL.2</italic> (10.36–12.97%), and <italic>QYrpd.swust-2BL.3</italic> (9.54–13.15%) were significant in some of the tests. The three ASR resistance QTLs <italic>QYrpd.swust-2AS</italic> (9.69–13.58%), <italic>QYrpd.swust-2BL.4</italic> (9.49–12.07%), and <italic>QYrpd.swust-7AS</italic> (16.16%) were detected based on the reactions in the seedlings tested with the CYR34 <italic>Pst</italic> race. Among the 15 QTLs detected in Pindong 34, the ASR resistance gene <italic>QYrpd.swust-7AS</italic> mapped on the short arm of chromosome 7A was likely similar to the previously reported QTL <italic>Yr61</italic> in the region. The QTLs identified in the present study and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.</p>