AUTHOR=Sun Meng , Liu Qi , Han Yi , Liu Guojun , Wu Jiajie , Qi Juan , Ni Fei , Bao Yinguang 

TITLE=PmSN15218: A Potential New Powdery Mildew Resistance Gene on Wheat Chromosome 2AL

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.931778

DOI=10.3389/fpls.2022.931778

ISSN=1664-462X

ABSTRACT=<p>Powdery mildew, caused by <italic>Blumeria graminis</italic> f. sp. <italic>tritici</italic> (<italic>Bgt</italic>), is a devastating fungal disease that seriously damages the yield and quality of wheat in many regions of the world. Identifying new resistance genes and breeding new resistant varieties are effective methods to control this disease. The breeding line SN15218 shows good resistance against powdery mildew. We, therefore, developed an F<sub>2</sub> population and 287 F<sub>2:3</sub> families crossed between SN15218 and the powdery mildew susceptible cultivar Huixianhong (HXH). Genetic analysis indicated that a single dominant gene, designated herein <italic>Pm</italic><sub><italic>SN15218</italic></sub>, conferred resistance to the <italic>Bgt</italic> isolate E09 in SN15218. Bulked segregant RNA-Seq (BSR-Seq) analysis revealed that <italic>Pm</italic><sub><italic>SN15218</italic></sub> is located in a ∼25-Mb interval on chromosome 2AL. Using the polymorphism information between SN15218 and HXH, we developed 13 polymerase chain reaction (PCR) markers and mapped this gene to a 0.5-cM genetic interval between the two flanking markers <italic>PmM12</italic> and <italic>PmM14</italic>, corresponding to a 6.01-Mb physical region in the Chinese Spring reference genome. The results of molecular marker analysis, allelic tests of resistance spectrum, and DNA resequencing indicated that <italic>Pm</italic><sub><italic>SN15218</italic></sub> is distinct from the known resistance gene <italic>Pm4b</italic> on 2AL.</p>