AUTHOR=Duan Yanling , Luo Jie , Yang Zujun , Li Guangrong , Tang Zongxiang , Fu Shulan 

TITLE=The Physical Location of Stripe Rust Resistance Genes on Chromosome 6 of Rye (Secale cereale L.) AR106BONE

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.928014

DOI=10.3389/fpls.2022.928014

ISSN=1664-462X

ABSTRACT=<p>It was reported that the chromosome 6R of rye (<italic>Secale cereale</italic> L.) carries stripe rust resistance gene <italic>Yr83</italic>, and the region with the candidate resistance gene(s) still needs to be narrowed down. This study confirmed that the chromosome 6RL<sup>Ar</sup> derived from rye AR106BONE contains stripe rust resistance gene(s). A wheat-rye T6BS.6RL<sup>Ar</sup> translocation chromosome, a wheat-rye small-segment translocation T6RL<sup>Ar</sup>-6AS.6AL, and three kinds of deleted T6BS.6RL<sup>Ar</sup> translocations, T6BS.6RL<sup>Ar</sup>-1, T6BS.6RL<sup>Ar</sup>-2, and T6BS.6RL<sup>Ar</sup>-3, were identified. Translocations T6BS.6RL<sup>Ar</sup>, T6BS.6RL<sup>Ar</sup>-2, and T6RL<sup>Ar</sup>-6AS.6AL were highly resistant to stripe rust and T6BS.6RL<sup>Ar</sup>-1 and T6BS.6RL<sup>Ar</sup>-3 were highly susceptible. The molecular markers specific to 6RL determined that the three regions of the 6RL<sup>Ar</sup> arm from 732,999,830 bp to the telomere, from 735,010,030 to 848,010,414 bp, and from 848,011,262 bp to the telomere were deleted from T6BS.6RL<sup>Ar</sup>-1, T6BS.6RL<sup>Ar</sup>-2, and T6BS.6RL<sup>Ar</sup>-3, respectively. T6BS.6RL<sup>Ar</sup>-2 and T6RL<sup>Ar</sup>-6AS.6AL contained the segment that was deleted in T6BS.6RL<sup>Ar</sup>-3. Therefore, it can be concluded that about 37 Mb segment from 848,011,262 bp to the telomere carried stripe rust resistance gene(s), and it was smaller than that with the <italic>Yr83</italic> gene. Gene annotation indicated that about 37 Mb region contains 43 potential resistance genes, and 42 of them are nucleotide-binding site and leucine-rich repeat (NBS-LRR)-like resistance protein genes. The results in this study narrowed down the size of the region with candidate stripe rust resistance gene(s) on the 6RL arm, and the T6RL<sup>Ar</sup>-6AS.6AL is a promising small-segment translocation for improvement of wheat cultivars.</p>