AUTHOR=Bai Bin , Li Zimeng , Wang Hongmei , Du Xiaolin , Wu Ling , Du Jiuyuan , Lan Caixia 

TITLE=Genetic Analysis of Adult Plant Resistance to Stripe Rust in Common Wheat Cultivar “Pascal”

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.918437

DOI=10.3389/fpls.2022.918437

ISSN=1664-462X

ABSTRACT=<p>Wheat stripe rust is an important foliar disease that affects the wheat yield globally. Breeding for resistant wheat varieties is one of the most economically and environmentally effective ways to control this disease. The common wheat (<italic>Triticum aestivum</italic> L.) cultivar “Pascal” exhibited susceptibility to stripe rust at the seedling stage but it showed high resistance to stripe rust at the adult plant stage over 20 years in Gansu, a hotspot of the disease in northwestern China. To understand the genetic mechanism of stripe rust resistance in this cultivar, a 55K SNP array was used to analyze the two parents and the 220 recombinant inbred lines (RILs) derived from the cross of “Huixianhong” × “Pascal.” We detected three new stripe rust adult plant resistance (APR) quantitative trait locus (QTL) contributed by Pascal, viz. <italic>QYr.gaas-1AL</italic>, <italic>QYr.gaas-3DL</italic>, and <italic>QYr.gaas-5AS</italic>, using the inclusive composite interval mapping method. They were flanked by SNP markers <italic>AX-111218361</italic>—<italic>AX-110577861</italic>, <italic>AX-111460455</italic>—<italic>AX-108798599</italic>, and <italic>AX-111523523</italic>—<italic>AX-110028503</italic>, respectively, and explained the phenotypic variation ranging from 11.0 to 23.1%. Bulked segregant exome capture sequencing (BSE-Seq) was used for fine mapping of <italic>QYr.gaas-1AL</italic> and selection of candidate genes, <italic>TraesCS1A02G313700</italic>, <italic>TraesCS1A02G313800</italic>, and <italic>TraesCS1A02G314900</italic> for <italic>QYr.gaas-1AL</italic>. KASP markers <italic>BSE-1A-12</italic> and <italic>HXPA-3D</italic> for <italic>QYr.gaas-1AL</italic> and <italic>QYr.gaas-3DL</italic> were developed for breeders to develop durable stripe rust-resistant wheat varieties.</p>