AUTHOR=Song Shiqiang , Zubov Dmitriy , Comes Hans Peter , Li Haiwen , Liu Xuelian , Zhong Xin , Lee Joongku , Yang Zhaoping , Li Pan 

TITLE=Plastid Phylogenomics and Plastome Evolution of Nandinoideae (Berberidaceae)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.913011

DOI=10.3389/fpls.2022.913011

ISSN=1664-462X

ABSTRACT=<p>Subfamily Nandinoideae Heintze (Berberidaceae), comprising four genera and <italic>ca.</italic> 19 species, is disjunctively distributed in eastern North America vs. Eurasia (eastern Asia, Central Asia, Middle East, and southeastern Europe), and represents an ideal taxon to explore plastid phylogenomics and plastome evolution in Berberidaceae. Many species of this subfamily have been listed as national or international rare and endangered plants. In this study, we sequenced and assembled 20 complete plastomes, representing three genera and 13 species of Nandinoideae. Together with six plastomes from GenBank, a total of 26 plastomes, representing all four genera and 16 species of Nandinoideae, were used for comparative genomic and phylogenomic analyses. These plastomes showed significant differences in overall size (156,626–161,406 bp), which is mainly due to the expansion in inverted repeat (IR) regions and/or insertion/deletion (indel) events in intergenic spacer (IGS) regions. A 75-bp deletion in the <italic>ndh</italic>F gene occurred in <italic>Leontice</italic> and <italic>Gymnospermium</italic> when compared with <italic>Nandina</italic> and <italic>Caulophyllum</italic>. We found a severe truncation at the 5’ end of <italic>ycf</italic>1 in three <italic>G. altaicum</italic> plastomes, and a premature termination of <italic>ropC</italic>1 in <italic>G. microrrhynchum</italic>. Our phylogenomic results support the topology of {<italic>Nandina</italic>, [<italic>Caulophyllum</italic>, (<italic>Leontice</italic>, <italic>Gymnospermium</italic>)]}. Within the core genus <italic>Gymnospermium</italic>, we identified <italic>G. microrrhynchum</italic> from northeastern Asia (Clade A) as the earliest diverging species, followed by <italic>G. kiangnanense</italic> from eastern China (Clade B), while the rest species clustered into the two sister clades (C and D). Clade C included three species from West Tianshan (<italic>G. albertii</italic>, <italic>G. darwasicum</italic>, <italic>G. vitellinum</italic>). Clade D consisted of <italic>G. altaicum</italic> from northern Central Asia, plus one species from the Caucasus Mountains (<italic>G. smirnovii</italic>) and three from southeastern Europe (<italic>G. odessanum</italic>, <italic>G. peloponnesiacum</italic>, <italic>G. scipetarum</italic>). Overall, we identified 21 highly variable plastome regions, including two coding genes (<italic>rpl</italic>22, <italic>ycf</italic>1) and 19 intergenic spacer (IGS) regions, all with nucleotide diversity (<italic>Pi</italic>) values &gt; 0.02. These molecular markers should serve as powerful tools (including DNA barcodes) for future phylogenetic, phylogeographic and conservation genetic studies.</p>