AUTHOR=Sajad Shoukat , Jiang Shuhan , Anwar Muhammad , Dai Qian , Luo Yuxia , Hassan Muhammad A. , Tetteh Charles , Song Jianghua 

TITLE=Genome-Wide Study of Hsp90 Gene Family in Cabbage (Brassica oleracea var. capitata L.) and Their Imperative Roles in Response to Cold Stress

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.908511

DOI=10.3389/fpls.2022.908511

ISSN=1664-462X

ABSTRACT=<p>Heat shock protein 90 (Hsp90) plays an important role in plant developmental regulation and defensive reactions. Several plant species have been examined for the <italic>Hsp90</italic> family gene. However, the <italic>Hsp90</italic> gene family in cabbage has not been well investigated to date. In this study, we have been discovered 12 <italic>BoHsp90</italic> genes in cabbage (<italic>Brassica oleracea</italic> var. <italic>capitata</italic> L.). These <italic>B. oleracea Hsp90</italic> genes were classified into five groups based on phylogenetic analysis. Among the five groups, group one contains five <italic>Hsp90</italic> genes, including <italic>BoHsp90-1</italic>, <italic>BoHsp90-2</italic>, <italic>BoHsp90-6</italic>, <italic>BoHsp90-10</italic>, and <italic>BoHsp90-12</italic>. Group two contains three <italic>Hsp90</italic> genes, including <italic>BoHsp90-3</italic>, <italic>BoHsp90-4</italic>, and <italic>BoHsp90</italic>. Group three only includes one <italic>Hsp90</italic> gene, including <italic>BoHsp90-9</italic>. Group four were consisting of three <italic>Hsp90</italic> genes including <italic>BoHsp90-5</italic>, <italic>BoHsp90-7</italic>, and <italic>BoHsp90-8</italic>, and there is no <italic>Hsp90</italic> gene from <italic>B. oleracea</italic> in the fifth group. Synteny analysis showed that a total of 12 <italic>BoHsp90</italic> genes have a collinearity relationship with 5 <italic>Arabidopsis</italic> genes and 10 <italic>Brassica rapa</italic> genes. The promoter evaluation revealed that the promoters of <italic>B. oleracea Hsp90</italic> genes included environmental stress-related and hormone-responsive <italic>cis-elements</italic>. RNA-seq data analysis indicates that tissue-specific expression of <italic>BoHsp90-9</italic> and <italic>BoHsp90-5</italic> were highly expressed in stems, leaves, silique, and flowers. Furthermore, the expression pattern of <italic>B. oleracea BoHsp90</italic> exhibited that <italic>BoHsp90-2, BoHsp90-3, BoHsp90-7, BoHsp90-9, BoHsp90-10, and BoHsp90-11</italic> were induced under cold stress, which indicates these <italic>Hsp90</italic> genes perform a vital role in cold acclimation and supports in the continual of normal growth and development process. The cabbage <italic>Hsp90</italic> gene family was found to be differentially expressed in response to cold stress, suggesting that these genes play an important role in cabbage growth and development under cold conditions.</p>