AUTHOR=Wang Chang , Liu Zhe , Wang Zicheng , Pang Wenhui , Zhang Long , Wen Zhaozhu , Zhao Yiran , Sun Juan , Wang Zeng-Yu , Yang Chao 

TITLE=Effects of autotoxicity and allelopathy on seed germination and seedling growth in Medicago truncatula

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.908426

DOI=10.3389/fpls.2022.908426

ISSN=1664-462X

ABSTRACT=<p>Autotoxicity is a form of intraspecific allelopathy, in which a plant species inhibits the establishment or growth of the same species through the release of toxic chemical compounds into the environment. The phenomenon of autotoxicity in crops is best traced in alfalfa (<italic>Medicago sativa</italic>). A close relative of alfalfa, <italic>M. truncatula,</italic> has been developed into an excellent model species for leguminous plants. However, it is not known whether <italic>M. truncatula</italic> has autotoxicity. In this study, <italic>M. truncatula</italic> root exudates showed a negative impact on the growth of <italic>M. truncatula</italic> seedlings, indicating autotoxicity. Detailed analyses with plant extracts from <italic>M. truncatula</italic> and alfalfa revealed varying degrees of suppression effects in the two species. The extracts negatively affected seed germination potential, germination rate, radicle length, hypocotyl length, synthetic allelopathic effect index, plant height, root growth, fresh weight, dry weight, net photosynthetic rate, transpiration rate, and stomatal conductance in both <italic>M. truncatula</italic> and alfalfa. The results demonstrated that autotoxicity and allelopathic effects exist in <italic>M. truncatula</italic>. This opens up a new way to use <italic>M. truncatula</italic> as a model species to carry out in-depth studies of autotoxicity and allelopathy to elucidate biochemical pathways of allelochemicals and molecular networks controlling biosynthesis of the chemicals.</p>