AUTHOR=Wang Chaohui , Wang Xueqin , Li Jinxiu , Guan Junhua , Tan Zengjing , Zhang Zheng , Shi Gangrong 

TITLE=Genome-Wide Identification and Transcript Analysis Reveal Potential Roles of Oligopeptide Transporter Genes in Iron Deficiency Induced Cadmium Accumulation in Peanut

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.894848

DOI=10.3389/fpls.2022.894848

ISSN=1664-462X

ABSTRACT=<p>The oligopeptide transporter (OPT) family is a group of proton-coupled symporters that play diverse roles, including metal homeostasis. However, little is known about this family of peanuts. To reveal the potential roles of <italic>AhOPT</italic> genes in Fe/Cd interactions, peanut <italic>AhOPT</italic> genes were genome-widely identified, and the relationships between gene expression and Cd accumulation were detected in two contrasting peanut cultivars (Fenghua 1 and Silihong) under Fe-sufficient or Fe-deficient conditions. A total of 40 <italic>AhOPT</italic> genes were identified in peanuts, which were divided into two subfamilies (PT and YS). Most <italic>AhOPT</italic> genes underwent gene duplication events predominated by whole-genome duplication. Clustered members generally have similar protein structures. However, gene structural divergences occurred in most of the duplicated genes. Transcription analysis revealed that <italic>AhOPT3.2</italic>/<italic>3.4</italic> and <italic>AhYSL3.1</italic>/<italic>3.2</italic> might be responsible for Fe deficiency tolerance, while <italic>AhOPT3.1</italic>/<italic>3.4, AhOPT7.1</italic>/<italic>7.2</italic>, and <italic>AhYSL1.1</italic> be involved in Fe/Cd interactions. These genes might be regulated by transcription factors, including <italic>ATHB-12, ATHB-6, DIVARICATA, MYB30, NAC02, DOF3.4, IDD7</italic>, and <italic>LUX</italic>. Reduced expressions of <italic>AhYSL3.1</italic>/<italic>3.2</italic> and higher expressions of <italic>AhOPT3.4</italic> might contribute to higher Fe-deficiency tolerance in Silihong. Higher expression of <italic>AhOPT7.3</italic> and <italic>AhOPT6.1</italic> might be responsible for low Cd accumulation in Fenghua 1. Our results confirmed that <italic>AhOPT3</italic>/<italic>6</italic>/<italic>7</italic> and <italic>AhYSL1</italic>/<italic>3</italic> might be involved in the transport of Fe and/or Cd in peanuts and provided new clues to understanding potential mechanisms of Fe/Cd interactions.</p>