AUTHOR=Lu Qing , Liu Hao , Hong Yanbin , Liang Xuanqiang , Li Shaoxiong , Liu Haiyan , Li Haifen , Wang Runfeng , Deng Quanqing , Jiang Huifang , Varshney Rajeev K. , Pandey Manish K. , Chen Xiaoping 

TITLE=Genome-Wide Identification and Expression of FAR1 Gene Family Provide Insight Into Pod Development in Peanut (Arachis hypogaea)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.893278

DOI=10.3389/fpls.2022.893278

ISSN=1664-462X

ABSTRACT=<p>The <italic>far-red-impaired response 1</italic> (<italic>FAR1</italic>) transcription family were initially identified as important factors for phytochrome A (phyA)-mediated far-red light signaling in <italic>Arabidopsis</italic>; they play crucial roles in controlling the growth and development of plants. The reported reference genome sequences of <italic>Arachis</italic>, including <italic>A. duranensis</italic>, <italic>A. ipaensis</italic>, <italic>A. monticola</italic>, and <italic>A. hypogaea</italic>, and its related species <italic>Glycine max</italic> provide an opportunity to systematically perform a genome-wide identification of <italic>FAR1</italic> homologous genes and investigate expression patterns of these members in peanut species. Here, a total of 650 <italic>FAR1</italic> genes were identified from four <italic>Aarchis</italic> and its closely related species <italic>G. max</italic>. Of the studied species, <italic>A. hypogaea</italic> contained the most (246) <italic>AhFAR1</italic> genes, which can be classified into three subgroups based on phylogenic relationships. The synonymous (<italic>Ks</italic>) and non-synonymous (<italic>Ka</italic>) substitution rates, phylogenetic relationship and synteny analysis of the <italic>FAR1</italic> family provided deep insight into polyploidization, evolution and domestication of peanut <italic>AhFAR1</italic> genes. The transcriptome data showed that the <italic>AhFAR1</italic> genes exhibited distinct tissue- and stage-specific expression patterns in peanut. Three candidate genes including <italic>Ahy_A10g049543</italic>, <italic>Ahy_A06g026579</italic>, and <italic>Ahy_A10g048401</italic>, specifically expressed in peg and pod, might participate in pod development in the peanut. The quantitative real-time PCR (qRT-PCR) analyses confirmed that the three selected genes were highly and specifically expressed in the peg and pod. This study systematically analyzed gene structure, evolutionary characteristics and expression patterns of <italic>FAR1</italic> gene family, which will provide a foundation for the study of genetic and biological function in the future.</p>