AUTHOR=Mbiza Natasha Isabel Tanatsiwa , Hu Zongwei , Zhang Haoran , Zhang Yi , Luo Xincheng , Wang Yuxue , Wang Yi , Liu Ting , Li Jianping , Wang Xiangping , Zhang Jianmin , Yu Yonghao 

TITLE=GhCalS5 is involved in cotton response to aphid attack through mediating callose formation

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.892630

DOI=10.3389/fpls.2022.892630

ISSN=1664-462X

ABSTRACT=<p>Callose synthase plays an essential role in plant growth and development and in response to all sorts of stresses through regulating callose formation. However, few research about the function and mechanism of the insect resistance of callose synthase genes have been reported in cotton. In this study, a cotton callose synthase gene <italic>GhCalS5</italic> was cloned, and its function and mechanism of resistance to cotton aphids were analyzed. The expression of <italic>GhCalS5</italic> was significantly upregulated in both, leaves and stems of cotton plants at 48 h after cotton aphid infestation and in the leaves of cotton plants at 24 h after salicylic acid treatment. The overexpression of <italic>GhCalS5</italic> enhanced cotton resistance to cotton aphids. Expectedly silencing of <italic>GhCalS5</italic> reduced cotton resistance to cotton aphids. Overexpression of <italic>GhCalS5</italic> enhanced callose formation in cotton leaves. Our results suggest that <italic>GhCalS5</italic> is involved in cotton resistance against cotton aphids by influencing callose formation.</p>