AUTHOR=Gao Yue , Qu Gaoyang , Huang Shengnan , Liu Zhiyong , Fu Wei , Zhang Meidi , Feng Hui 

TITLE=BrCPS1 Function in Leafy Head Formation Was Verified by Two Allelic Mutations in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.889798

DOI=10.3389/fpls.2022.889798

ISSN=1664-462X

ABSTRACT=<p>The formation of the leafy heads of Chinese cabbage is an important agricultural factor because it directly affects yield. In this study, we identified two allelic non-heading mutants, <italic>nhm4-1</italic> and <italic>nhm4-2</italic>, from an ethyl methanesulfonate mutagenic population of a heading Chinese cabbage double haploid line “FT.” Using MutMap, Kompetitive Allele-Specific PCR genotyping, and map-based cloning, we found that <italic>BraA09g001440.3C</italic> was the causal gene for the mutants. <italic>BraA09g001440.3C</italic> encodes an <italic>ent</italic>-copalyl diphosphate synthase 1 involved in gibberellin biosynthesis. A single non-synonymous SNP in the seventh and fourth exons of <italic>BraA09g001440.3C</italic> was responsible for the <italic>nhm4-1</italic> and <italic>nhm4-2</italic> mutant phenotypes, respectively. Compared with the wild-type “FT,” the gibberellin content in the mutant leaves was significantly reduced. Both mutants showed a tendency to form leafy heads after exogenous GA<sub>3</sub> treatment. The two non-heading mutants and the work presented herein demonstrate that gibberellin is related to leafy head formation in Chinese cabbage.</p>