AUTHOR=Duan Bing , Du Huaying , Zhang Wei , Wang Jing , Cai Zhipeng , Shen Yonggen , Huang Tenghuan , Yuan Jie , Gan Zengyu , Chen Jinyin , Zhu Liqin 

TITLE=An Antifungal Role of Hydrogen Sulfide on Botryosphaeria Dothidea and Amino Acid Metabolism Involved in Disease Resistance Induced in Postharvest Kiwifruit

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.888647

DOI=10.3389/fpls.2022.888647

ISSN=1664-462X

ABSTRACT=<p><italic>Botryosphaeria dothidea</italic> is a major pathogen responsible for postharvest kiwifruit soft rot. This study aimed to determine the influence of hydrogen sulfide (H<sub>2</sub>S) on postharvest resistance to kiwifruit soft rot and the antifungal role of H<sub>2</sub>S against <italic>B. dothidea</italic>. The results indicated that H<sub>2</sub>S (20 μl L<sup>−1</sup>) restricted the lesion area following inoculation with <italic>B. dothidea</italic>. H<sub>2</sub>S enhanced the production of shikimic acid, tyrosine, tryptophan, and phenylalanine while also increasing the total phenols, flavonoids, and lignin. H<sub>2</sub>S upregulated the expression of <italic>AcDHQS, AcSDH, AcSK, AcPAL, AcCAD</italic>, and <italic>AcCHS</italic>. Additionally, sodium hydrosulfide (NaHS)-released H<sub>2</sub>S inhibited mycelial growth. NaHS concentrations of 20 and 40 mmol L<sup>−1</sup> significantly decreased the mycelial weight and malondialdehyde content (MDA) content while increasing cell membrane conductivity and membrane leakage. The results indicate that H<sub>2</sub>S induces resistance in kiwifruit <italic>via</italic> a microbicidal role and amino acid metabolism involved in postharvest kiwifruit disease resistance.</p>