AUTHOR=Bull Tawni , Michelmore Richard 

TITLE=Molecular Determinants of in vitro Plant Regeneration: Prospects for Enhanced Manipulation of Lettuce (Lactuca sativa L.)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.888425

DOI=10.3389/fpls.2022.888425

ISSN=1664-462X

ABSTRACT=<p><italic>In vitro</italic> plant regeneration involves dedifferentiation and molecular reprogramming of cells in order to regenerate whole organs. Plant regeneration can occur via two pathways, <italic>de novo</italic> organogenesis and somatic embryogenesis. Both pathways involve intricate molecular mechanisms and crosstalk between auxin and cytokinin signaling. Molecular determinants of both pathways have been studied in detail in model species, but little is known about the molecular mechanisms controlling <italic>de novo</italic> shoot organogenesis in lettuce. This review provides a synopsis of our current knowledge on molecular determinants of <italic>de novo</italic> organogenesis and somatic embryogenesis with an emphasis on the former as well as provides insights into applying this information for enhanced <italic>in vitro</italic> regeneration in non-model species such as lettuce (<italic>Lactuca sativa</italic> L.).</p>