AUTHOR=Dai Jiahao , Sun Jin , Peng Wenjing , Liao Wenhai , Zhou Yuhan , Zhou Xue-Rong , Qin Yuan , Cheng Yan , Cao Shijiang 

TITLE=FAR1/FHY3 Transcription Factors Positively Regulate the Salt and Temperature Stress Responses in Eucalyptus grandis

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.883654

DOI=10.3389/fpls.2022.883654

ISSN=1664-462X

ABSTRACT=<p>FAR-RED ELONGATED HYPOCOTYLS3 (FHY3) and its homolog FAR-RED IMPAIRED RESPONSE1 (FAR1), which play pivotal roles in plant growth and development, are essential for the photo-induced phyA nuclear accumulation and subsequent photoreaction. The FAR1/FHY3 family has been systematically characterized in some plants, but not in <italic>Eucalyptus grandis</italic>. In this study, genome-wide identification of <italic>FAR1/FHY3</italic> genes in <italic>E. grandis</italic> was performed using bioinformatic methods. The gene structures, chromosomal locations, the encoded protein characteristics, 3D models, phylogenetic relationships, and promoter <italic>cis</italic>-elements were analyzed with this gene family. A total of 33 <italic>FAR1/FHY3</italic> genes were identified in <italic>E. grandis</italic>, which were divided into three groups based on their phylogenetic relationships. A total of 21 pairs of duplicated repeats were identified by homology analysis. Gene expression analysis showed that most <italic>FAR1/FHY3</italic> genes were differentially expressed in a spatial-specific manner. Gene expression analysis also showed that <italic>FAR1/FHY3</italic> genes responded to salt and temperature stresses. These results and observation will enhance our understanding of the evolution and function of the <italic>FAR1/FHY3</italic> genes in <italic>E. grandis</italic> and facilitate further studies on the molecular mechanism of the <italic>FAR1/FHY3</italic> gene family in growth and development regulations, especially in response to salt and temperature.</p>