AUTHOR=Yang Peng , Sun Xiaoting , Liu Xueying , Wang Wenwen , Hao Yongshui , Chen Lei , Liu Jun , He Hailun , Zhang Taorui , Bao Wanyu , Tang Yihua , He Xinran , Ji Mengya , Guo Kai , Liu Dexin , Teng Zhonghua , Liu Dajun , Zhang Jian , Zhang Zhengsheng 

TITLE=Identification of Candidate Genes for Lint Percentage and Fiber Quality Through QTL Mapping and Transcriptome Analysis in an Allotetraploid Interspecific Cotton CSSLs Population

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.882051

DOI=10.3389/fpls.2022.882051

ISSN=1664-462X

ABSTRACT=<p>Upland cotton (<italic>Gossypium hirsutum</italic>) has long been an important fiber crop, but the narrow genetic diversity of modern <italic>G. hirsutum</italic> limits the potential for simultaneous improvement of yield and fiber quality. It is an effective approach to broaden the genetic base of <italic>G. hirsutum</italic> through introgression of novel alleles from <italic>G. barbadense</italic> with excellent fiber quality. In the present study, an interspecific chromosome segment substitution lines (CSSLs) population was established using <italic>G. barbadense</italic> cultivar Pima S-7 as the donor parent and <italic>G. hirsutum</italic> cultivar CCRI35 as the recipient parent. A total of 105 quantitative trait loci (QTL), including 85 QTL for fiber quality and 20 QTL for lint percentage (LP), were identified based on phenotypic data collected from four environments. Among these QTL, 25 stable QTL were detected in two or more environments, including four for LP, eleven for fiber length (FL), three for fiber strength (FS), six for fiber micronaire (FM), and one for fiber elongation (FE). Eleven QTL clusters were observed on nine chromosomes, of which seven QTL clusters harbored stable QTL. Moreover, eleven major QTL for fiber quality were verified through analysis of introgressed segments of the eight superior lines with the best comprehensive phenotypes. A total of 586 putative candidate genes were identified for 25 stable QTL associated with lint percentage and fiber quality through transcriptome analysis. Furthermore, three candidate genes for FL, <italic>GH_A08G1681</italic> (<italic>GhSCPL40</italic>), <italic>GH_A12G2328</italic> (<italic>GhPBL19</italic>), and <italic>GH_D02G0370</italic> (<italic>GhHSP22.7</italic>), and one candidate gene for FM, <italic>GH_D05G1346</italic> (<italic>GhAPG</italic>), were identified through RNA-Seq and qRT-PCR analysis. These results lay the foundation for understanding the molecular regulatory mechanism of fiber development and provide valuable information for marker-assisted selection (MAS) in cotton breeding.</p>