AUTHOR=Hu Peng , Tan Yiqing , Wen Yi , Fang Yunxia , Wang Yueying , Wu Hao , Wang Junge , Wu Kaixiong , Chai Bingze , Zhu Li , Zhang Guangheng , Gao Zhenyu , Ren Deyong , Zeng Dali , Shen Lan , Xue Dawei , Qian Qian , Hu Jiang 

TITLE=LMPA Regulates Lesion Mimic Leaf and Panicle Development Through ROS-Induced PCD in Rice

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.875038

DOI=10.3389/fpls.2022.875038

ISSN=1664-462X

ABSTRACT=<p>Leaf and panicle are important nutrient and yield organs in rice, respectively. Although several genes controlling lesion mimic leaf and panicle abortion have been identified, a few studies have reported the involvement of a single gene in the production of both the traits. In this study, we characterized a panicle abortion mutant, <italic>lesion mimic leaf and panicle apical abortion</italic> (<italic>lmpa</italic>), which exhibits lesions on the leaf and causes degeneration of apical spikelets. Molecular cloning revealed that <italic>LMPA</italic> encodes a proton pump ATPase protein that is localized in the plasma membrane and is highly expressed in leaves and panicles. The analysis of promoter activity showed that the insertion of a fragment in the promoter of <italic>lmpa</italic> caused a decrease in the transcription level. Cellular and histochemistry analysis indicated that the ROS accumulated and cell death occurred in lmpa. Moreover, physiological experiments revealed that <italic>lmpa</italic> was more sensitive to high temperatures and salt stress conditions. These results provide a better understanding of the role of <italic>LMPA</italic> in panicle development and lesion mimic formation by regulating ROS homeostasis.</p>