AUTHOR=Zhang Lihua , Yang Yi , Mu Changqing , Liu Mingyu , Ishida Takashi , Sawa Shinichiro , Zhu Yuxian , Pi Limin 

TITLE=Control of Root Stem Cell Differentiation and Lateral Root Emergence by CLE16/17 Peptides in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.869888

DOI=10.3389/fpls.2022.869888

ISSN=1664-462X

ABSTRACT=<p>Secreted peptide-mediated cell-to-cell communication plays a crucial role in the development of multicellular organisms. A large number of secreted peptides have been predicated by bioinformatic approaches in plants. However, only a few of them have been functionally characterized. In this study, we show that two CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides CLE16/17 are required for both stem cell differentiation and lateral root (LR) emergence in <italic>Arabidopsis</italic>. We further demonstrate that the CLE16/17 peptides act through the CLAVATA1-ARABIDOPSIS CRINKLY4 (CLV1-ACR4) protein kinase complex in columella stem cell (CSC) differentiation, but not in LR emergence. Furthermore, we show that CLE16/17 promote LR emergence probably <italic>via</italic> activating the expression of <italic>HAESA</italic>/<italic>HAESA-LIKE2</italic> (<italic>HAE</italic>/<italic>HSL2</italic>) required for cell wall remodeling. Collectively, our results reveal a CLV1-ACR4-dependent and -independent dual-function of the CLE16/17 peptides in root development.</p>