AUTHOR=Li Qingqing , Liu Chaobo , Huang Ceyin , Wang Mufei , Long Teng , Liu Jingyi , Shi Junhua , Shi Junli , Li Lin , He Yuqi , Xu De-Lin 

TITLE=Transcriptome and Metabonomics Analysis Revealed the Molecular Mechanism of Differential Metabolite Production of Dendrobium nobile Under Different Epiphytic Patterns

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.868472

DOI=10.3389/fpls.2022.868472

ISSN=1664-462X

ABSTRACT=<p>The cultivation medium of <italic>Dendrobium nobile</italic> has an effect on the contents of its main medicinal components, but the specific mechanism is still unclear. In this study, the callus, seedlings, rhizomes, and leaves of <italic>D. nobile</italic> were sequenced for the PacBio SMRT. The 2-year-old stems were selected for the Illumina sequencing and metabolome sequencing to analyze the genetic mechanism of metabolic differences under different epiphytic patterns. As a result, a total of 387 differential genes were obtained, corresponding to 66 differential metabolites. Different epiphytic patterns can induce a series of metabolic changes at the metabolome and transcriptome levels of <italic>D. nobile</italic>, including flavonoid metabolism, purine metabolism, terpenoid backbone biosynthesis, amino acid metabolism, and alpha-linolenic acid metabolic, and related regulatory genes include <italic>ALDH2B7, ADC, EPSPS-1, SHKA, DHAPS-1, GES, ACS1, SAHH, ACS2, CHLP, LOX2, LOX2.3</italic>, and <italic>CYP74B2</italic>. The results showed that the genetic mechanism of <italic>D. nobile</italic> under various epiphytic patterns was different. In theory, the content of metabolites under the epiphytic patterns of Danxia stone is higher, which is more suitable for field cultivation.</p>