AUTHOR=Zhang Qing , Deng Aiwen , Xiang Min , Lan Qiuyan , Li Xiaokun , Yuan Shuai , Gou Xin , Hao Shuang , Du Juan , Xiao Chaowen 

TITLE=The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.862171

DOI=10.3389/fpls.2022.862171

ISSN=1664-462X

ABSTRACT=<p>Pectin, cellulose, and hemicellulose constitute the primary cell wall in eudicots and function in multiple developmental processes in plants. Root hairs are outgrowths of specialized epidermal cells that absorb water and nutrients from the soil. Cell wall architecture influences root hair development, but how cell wall remodeling might enable enhanced root hair formation in response to phosphate (P) deficiency remains relatively unclear. Here, we found that POLYGALACTURONASE INVOLVED IN EXPANSION 2 (PGX2) functions in conditional root hair development. Under low P conditions, a <italic>PGX2</italic> activation tagged line (<italic>PGX2<sup>AT</sup></italic>) displays bubble-like root hairs and abnormal callose deposition and superoxide accumulation in roots. We found that the polar localization and trafficking of PIN2 are altered in <italic>PGX2<sup>AT</sup></italic> roots in response to P deficiency. We also found that actin filaments were less compact but more stable in <italic>PGX2<sup>AT</sup></italic> root hair cells and that actin filament skewness in <italic>PGX2<sup>AT</sup></italic> root hairs was recovered by treatment with 1-N-naphthylphthalamic acid (NPA), an auxin transport inhibitor. These results demonstrate that activation tagging of <italic>PGX2</italic> affects cell wall remodeling, auxin signaling, and actin microfilament orientation, which may cooperatively regulate root hair development in response to P starvation.</p>