AUTHOR=Guo Xiuna , Chen Jinyin , Gao Mengyi , Li Duochuan 

TITLE=An Aminobutyric Acid Transaminase in Zea mays Interacts With Rhizoctonia solani Cellulase to Participate in Disease Resistance

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.860170

DOI=10.3389/fpls.2022.860170

ISSN=1664-462X

ABSTRACT=<p>Corn sheath blight, caused by AG1-IA, a fusion group of <italic>Rhizoctonia solani</italic>, which acts as a kind of necrotrophic fungal pathogen, poses a global threat to the production of <italic>Zea mays</italic>. Although cellulase plays a crucial role in <italic>R. solani</italic> infections, how plants respond to it is still poorly understood. In this study, we identified a gamma-aminobutyric acid transaminase (GABA-T), ZmGABA-T, in <italic>Z. mays</italic> that interacts with a cell wall–degrading enzyme (CWDE), EG1, in the cell membrane, using yeast two-hybrid assay, co-immunoprecipitation (Co-IP), and bimolecular fluorescence complementation assays. We found that the combination of EG1 and ZmGABA-T suppressed the allergic necrosis induced by EG1. We also found that the substrate of GABA-T–GABA, can inhibit the transcription of EG1. Transient expression of ZmGABA-T inhibited <italic>R. solani</italic> infection in <italic>Nicotiana benthamiana</italic>. The homolog in <italic>Oryza sativa</italic>, OsGABA-T, could also interact with EG1 to suppress the allergic necrosis induced by EG1. The OsGABA-T knocked out plants displayed enhanced susceptibility to <italic>R. solani</italic> and showed larger lesions. In conclusion, our results suggest that ZmGABA-T inhibits allergic necrosis induced by EG1 based on the combination with EG1, producing resistance to <italic>R. solani</italic> infection.</p>