AUTHOR=Mangel Nathalie , Fudge Jared B. , Gruissem Wilhelm , Fitzpatrick Teresa B. , Vanderschuren Hervé 

TITLE=Natural Variation in Vitamin B1 and Vitamin B6 Contents in Rice Germplasm

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.856880

DOI=10.3389/fpls.2022.856880

ISSN=1664-462X

ABSTRACT=<p>Insufficient dietary intake of micronutrients contributes to the onset of deficiencies termed hidden hunger—a global health problem affecting approximately 2 billion people. Vitamin B<sub>1</sub> (thiamine) and vitamin B<sub>6</sub> (pyridoxine) are essential micronutrients because of their roles as enzymatic cofactors in all organisms. Metabolic engineering attempts to biofortify rice endosperm—a poor source of several micronutrients leading to deficiencies when consumed monotonously—have led to only minimal improvements in vitamin B<sub>1</sub> and B<sub>6</sub> contents. To determine if rice germplasm could be exploited for biofortification of rice endosperm, we screened 59 genetically diverse accessions under greenhouse conditions for variation in vitamin B<sub>1</sub> and vitamin B<sub>6</sub> contents across three tissue types (leaves, unpolished and polished grain). Accessions from low, intermediate and high vitamin categories that had similar vitamin levels in two greenhouse experiments were chosen for in-depth vitamer profiling and selected biosynthesis gene expression analyses. Vitamin B<sub>1</sub> and B<sub>6</sub> contents in polished seeds varied almost 4-fold. Genes encoding select vitamin B<sub>1</sub> and B<sub>6</sub> biosynthesis <italic>de novo</italic> enzymes (<italic>THIC</italic> for vitamin B<sub>1</sub>, <italic>PDX1.3a</italic>–<italic>c</italic> and <italic>PDX2</italic> for vitamin B<sub>6</sub>) were differentially expressed in leaves across accessions contrasting in their respective vitamin contents. These expression levels did not correlate with leaf and unpolished seed vitamin contents, except for <italic>THIC</italic> expression in leaves that was positively correlated with total vitamin B<sub>1</sub> contents in polished seeds. This study expands our knowledge of diversity in micronutrient traits in rice germplasm and provides insights into the expression of genes for vitamin B<sub>1</sub> and B<sub>6</sub> biosynthesis in rice.</p>