AUTHOR=Huang Feiyi , Shi Churan , Zhang Yuhang , Hou Xilin 

TITLE=Genome-Wide Identification and Characterization of TCP Family Genes in Pak-Choi [Brassica campestris (syn. Brassica rapa) ssp. chinensis var. communis]

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.854171

DOI=10.3389/fpls.2022.854171

ISSN=1664-462X

ABSTRACT=<p>The TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) gene family, a kind of plant specific transcription factor, is essential for stress response, cell growth, and cell proliferation. However, the characterization of TCP family is still not clear in Pak-choi [<italic>Brassica campestris</italic> (syn. <italic>Brassica rapa</italic>) ssp. <italic>chinensis</italic> var. <italic>communis</italic>]. In this study, genome-wide analysis of TCP gene family was performed and 26 <italic>TCP</italic> genes were identified in Pak-choi. Phylogenetic analysis demonstrated that the 26 BcTCPs were divided into two classes: Class I and Class II. Class II was further classified into two subclasses, CIN and CYC/TB1. The qPCR results suggested that most <italic>BcTCPs</italic> respond to abiotic stresses. The expressions of <italic>BcTCP3</italic>, <italic>BcTCP12</italic>, <italic>BcTCP21</italic>, and <italic>BcTCP22</italic> were significantly changed under ABA and cold treatment. <italic>BcTCP3</italic> and <italic>BcTCP12</italic> were also up-regulated under osmotic treatment. Subcellular localization showed that BcTCP3 and BcTCP21 were located in the nucleus. Our results will facilitate revealing the functions and regulatory mechanisms of <italic>BcTCPs</italic>.</p>