AUTHOR=Chen Qinchang , Zhang Xiaodan , Fang Yaxue , Wang Baiyu , Xu Shaosi , Zhao Kai , Zhang Jisen , Fang Jingping 

TITLE=Genome-Wide Identification and Expression Analysis of the R2R3-MYB Transcription Factor Family Revealed Their Potential Roles in the Flowering Process in Longan (Dimocarpus longan)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.820439

DOI=10.3389/fpls.2022.820439

ISSN=1664-462X

ABSTRACT=<p>Longan (<italic>Dimocarpus longan</italic> Lour.) is a productive fruit crop with high nutritional and medical value in tropical and subtropical regions. The <italic>MYB</italic> gene family is one of the most widespread plant transcription factor (TF) families participating in the flowering regulation. However, little is known about the MYB TFs involved in the flowering process in longan and its regulatory network. In this study, a total of 119 <italic>DlR2R3-MYB</italic> genes were identified in the longan genome and were phylogenetically grouped into 28 subgroups. The groupings were supported by highly conserved gene structures and motif composition of <italic>DlR2R3-MYB</italic> genes in each subgroup. Collinearity analysis demonstrated that segmental replications played a more crucial role in the expansion of the <italic>DlR2R3-MYB</italic> gene family compared to tandem duplications, and all tandem/segmental duplication gene pairs have evolved under purifying selection. Interspecies synteny analysis among longan and five representative species implied the occurrence of gene duplication events was one of the reasons contributing to functional differentiation among species. RNA-seq data from various tissues showed <italic>DlR2R3-MYB</italic> genes displayed tissue-preferential expression patterns. The pathway of flower development was enriched with six <italic>DlR2R3-MYB</italic> genes. <italic>Cis</italic>-acting element prediction revealed the putative functions of <italic>DlR2R3-MYB</italic> genes were related to the plant development, phytohormones, and environmental stresses. Notably, the orthologous counterparts between Arabidopsis and longan R2R3-MYB members tended to play conserved roles in the flowering regulation and stress responses. Transcriptome profiling on off-season flower induction (FI) by KClO<sub>3</sub> indicated two up-regulated and four down-regulated <italic>DlR2R3-MYB</italic> genes involved in the response to KClO<sub>3</sub> treatment compared with control groups. Additionally, qRT-PCR confirmed certain genes exhibited high expression in flowers/flower buds. Subcellular localization experiments revealed that three predicted flowering-associated MYB proteins were localized in the nucleus. Future functional studies on these potential candidate genes involved in the flowering development could further the understanding of the flowering regulation mechanism.</p>