AUTHOR=Han Wanli , Zhao Jieyin , Deng Xiaojuan , Gu Aixing , Li Duolu , Wang Yuxiang , Lu Xiaoshuang , Zu Qianli , Chen Qin , Chen Quanjia , Zhang Jinfa , Qu Yanying 

TITLE=Quantitative Trait Locus Mapping and Identification of Candidate Genes for Resistance to Fusarium Wilt Race 7 Using a Resequencing-Based High Density Genetic Bin Map in a Recombinant Inbred Line Population of Gossypium barbadense

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.815643

DOI=10.3389/fpls.2022.815643

ISSN=1664-462X

ABSTRACT=<p>Fusarium wilt caused by <italic>Fusarium oxysporum</italic> f. sp. <italic>vasinfectum</italic> (FOV) is one of the most destructive diseases in cotton (<italic>Gossypium</italic> spp.) production, and use of resistant cultivars is the most cost-effective method managing the disease. To understand the genetic basis of cotton resistance to FOV race 7 (FOV7), this study evaluated a recombinant inbred line (RIL) population of 110 lines of <italic>G. barbadense</italic> from a cross between susceptible Xinhai 14 and resistant 06-146 in eight tests and constructed a high-density genetic linkage map with resequencing-based 933,845 single-nucleotide polymorphism (SNP) markers covering a total genetic distance of 2483.17 cM. Nine quantitative trait loci (QTLs) for FOV7 resistance were identified, including <italic>qFOV7-D03-1</italic> on chromosome D03 in two tests. Through a comparative analysis of gene expression and DNA sequence for predicted genes within the QTL region between the two parents and selected lines inoculated with FOV7, <italic>GB_D03G0217</italic> encoding for a calmodulin (CaM)-like (CML) protein was identified as a candidate gene. A further analysis confirmed that the expression of <italic>GB_D03G0217</italic> was suppressed, leading to increased disease severity in plants of the resistant parent with virus induced gene silencing (VIGS).</p>