AUTHOR=Liu Hongru , Cao Xiangmei , Azam Muhammad , Wang Chunfang , Liu Chenxia , Qiao Yongjin , Zhang Bo 

TITLE=Metabolism of Carotenoids and β-Ionone Are Mediated by Carotenogenic Genes and PpCCD4 Under Ultraviolet B Irradiation and During Fruit Ripening

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.814677

DOI=10.3389/fpls.2022.814677

ISSN=1664-462X

ABSTRACT=<p>Carotenoids are essential pigments widely distributed in tissues and organs of higher plants, contributing to color, photosynthesis, photoprotection, nutrition, and flavor in plants. White- or yellow-fleshed colors in peach were determined by expression of carotenoids cleavage dioxygenase (<italic>PpCCD</italic>) genes, catalyzing the degradation of carotenoids. The cracked volatile apocarotenoids are the main contributors to peach aroma and flavor with low sensory threshold concentration. However, the detailed regulatory roles of carotenoids metabolism genes remained unclear under UV-B irradiation. In our study, metabolic balance between carotenoids and apocarotenoids was regulated by the expression of phytoene synthase (<italic>PSY</italic>), β-cyclase (<italic>LCY-B</italic>), ε<italic>-</italic>cyclase (<italic>LCY-E</italic>), and <italic>PpCCD4</italic> under UV-B irradiation. The transcript levels of <italic>PpPSY</italic>, <italic>PpLCY-B</italic>, <italic>PpLCY-E</italic>, and <italic>PpCHY-B</italic> were elevated 2- to 10-fold compared with control, corresponding to a nearly 30% increase of carotenoids content after 6 h UV-B irradiation. Interestingly, the total carotenoids content decreased by nearly 60% after 48 h of storage, while UV-B delayed the decline of lutein and β-carotene. The transcript level of <italic>PpLCY-E</italic> increased 17.83-fold compared to control, partially slowing the decline rate of lutein under UV-B irradiation. In addition, the transcript level of <italic>PpCCD4</italic> decreased to 30% of control after 48 h UV-B irradiation, in accordance with the dramatic reduction of apocarotenoid volatiles and the delayed decrease of β-carotene. Besides, β-ionone content was elevated by ethylene treatment, and accumulation dramatically accelerated at full ripeness. Taken together, UV-B radiation mediated the metabolic balance of carotenoid biosynthesis and catabolism by controlling the transcript levels of <italic>PpPSY</italic>, <italic>PpLCY-B</italic>, <italic>PpLCY-E</italic>, and <italic>PpCCD4</italic> in peach, and the transcript level of <italic>PpCCD4</italic> showed a positive relationship with the accumulation of β-ionone during the ripening process. However, the detailed catalytic activity of PpCCD4 with various carotenoid substrates needs to be studied further, and the key transcript factors involved in the regulation of metabolism between carotenoids and apocarotenoids need to be clarified.</p>