AUTHOR=Kim Jin A. , Moon Heewon , Kim Hyang Suk , Choi Dasom , Kim Nan-Sun , Jang Juna , Lee Sang Woo , Baskoro Dwi Nugroho Adji , Kim Dong-Hwan 

TITLE=Transcriptome and QTL mapping analyses of major QTL genes controlling glucosinolate contents in vegetable- and oilseed-type Brassica rapa plants

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1067508

DOI=10.3389/fpls.2022.1067508

ISSN=1664-462X

ABSTRACT=<p>Glucosinolates (GSLs) are secondary metabolites providing defense against pathogens and herbivores in plants, and anti-carcinogenic activity against human cancer cells. Profiles of GSLs vary greatly among members of genus <italic>Brassica</italic>. In this study, we found that a reference line of Chinese cabbage (<italic>B. rapa</italic> ssp. <italic>pekinensis</italic>), ‘Chiifu’ contains significantly lower amounts of total GSLs than the oilseed-type <italic>B. rapa</italic> (<italic>B. rapa</italic> ssp. <italic>trilocularis</italic>) line ‘LP08’. This study aimed to identify the key regulators of the high accumulation of GSLs in <italic>Brassica rapa</italic> plants using transcriptomic and linkage mapping approaches. Comparative transcriptome analysis showed that, in total, 8,276 and 9,878 genes were differentially expressed between ‘Chiifu’ and ‘LP08’ under light and dark conditions, respectively. Among 162 <italic>B. rapa</italic> GSL pathway genes, 79 were related to GSL metabolism under light conditions. We also performed QTL analysis using a single nucleotide polymorphism-based linkage map constructed using 151 F<sub>5</sub> individuals derived from a cross between the ‘Chiifu’ and ‘LP08’ inbred lines. Two major QTL peaks were successfully identified on chromosome 3 using high-performance liquid chromatography to obtain GSL profiles from 97 F<sub>5</sub> recombinant inbred lines. The MYB-domain transcription factor gene <italic>BrMYB28.1</italic> (Bra012961) was found in the highest QTL peak region. The second highest peak was located near the 2-oxoacid-dependent dioxygenase gene <italic>BrGSL-OH.1</italic> (Bra022920). This study identified major genes responsible for differing profiles of GSLs between ‘Chiifu’ and ‘LP08’. Thus, our study provides molecular insights into differences in GSL profiles between vegetative- and oilseed-type <italic>B. rapa</italic> plants.</p>