AUTHOR=Jose Jeny , Éva Csaba , Bozsó Zoltán , Hamow Kamirán Áron , Fekete Zsófia , Fábián Attila , Bánfalvi Zsófia , Sági László 

TITLE=Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1065419

DOI=10.3389/fpls.2022.1065419

ISSN=1664-462X

ABSTRACT=<p><italic>Ralstonia solanacearum</italic> (<italic>Rs</italic>), the causal agent of bacterial wilt disease in an unusually wide range of host plants, including potato (<italic>Solanum tuberosum</italic>), is one of the most destructive phytopathogens that seriously reduces crop yields worldwide. Identification of defence mechanisms underlying bacterial wilt resistance is a prerequisite for biotechnological approaches to resistance breeding. Resistance to <italic>Rs</italic> has been reported only in a few potato landraces and cultivars. Our <italic>in vitro</italic> inoculation bioassays confirmed that the cultivars ‘Calalo Gaspar’ (CG) and ‘Cruza 148’ (CR) are resistant to <italic>Rs</italic> infection. Comparative transcriptome analyses of CG and CR roots, as well as of the roots of an <italic>Rs</italic>-susceptible cultivar, ‘Désirée’ (DES), were carried out two days after <italic>Rs</italic> infection, in parallel with their respective noninfected controls. In CR and DES, the upregulation of chitin interactions and cell wall-related genes was detected. The phenylpropanoid biosynthesis and glutathione metabolism pathways were induced only in CR, as confirmed by high levels of lignification over the whole stele in CR roots six days after <italic>Rs</italic> infection. At the same time, <italic>Rs</italic> infection greatly increased the concentrations of chlorogenic acid and quercetin derivatives in CG roots as it was detected using ultra-performance liquid chromatography − tandem mass spectrometry. Characteristic increases in the expression of MAP kinase signalling pathway genes and in the concentrations of jasmonic, salicylic, abscisic and indoleacetic acid were measured in DES roots. These results indicate different <italic>Rs</italic> defence mechanisms in the two resistant potato cultivars and a different response to <italic>Rs</italic> infection in the susceptible cultivar.</p>