AUTHOR=Zhang Xueying , Li Linying , Lang Zhuoliang , Li Da , He Yuqing , Zhao Yao , Tao Han , Wei Jiqian , Li Qingsheng , Hong Gaojie 

TITLE=Genome-wide characterization of NAC transcription factors in Camellia sinensis and the involvement of CsNAC28 in drought tolerance

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1065261

DOI=10.3389/fpls.2022.1065261

ISSN=1664-462X

ABSTRACT=<p>The NAM, ATAF1/2, and CUC2 (NAC) transcription factors, which are members of a plant-specific gene family, play critical roles during the growth and development of plants and in their adaption to environmental stress. Few NAC transcription factors have been functionally characterized in tea plants (<italic>Camellia sinensis</italic>). Based on the analysis of the gene structure, motif pattern, and evolutionary relationship, we identified 104 NAC genes in <italic>C. sinensis</italic>. Among them, <italic>CsNAC28</italic> is constitutively expressed in all organs, and most significantly, exhibiting remarkable responsiveness to abscisic acid (ABA) treatment and drought stress. ABA is a primary stress-related hormone. Recently, ABA-responsive element binding factor 2 (<italic>CsABF2</italic>) was identified in the ABA pathway of <italic>C. sinensis.</italic> However, the involvement of the <italic>CsABF2</italic>-mediated ABA pathway in regulating CsNACs was not known. Herein, a series of biochemical and genetic approaches supported the fact that <italic>CsNAC28</italic> could potentially act as a transcription factor in the downstream of <italic>CsABF2</italic>. Furthermore, we investigated the function of <italic>CsNAC28</italic> in the adapting of a plant to drought stress. The results showed that overexpression of <italic>CsNAC28</italic> in Arabidopsis conferred hypersensitivity to ABA treatment and decreased the accumulation of reactive oxygen species (ROS), resulting in improved dehydration tolerance. Under conditions of drought, the expression levels of ABA pathway-related genes and drought stress‒inducible genes were greater in <italic>CsNAC28</italic> overexpression lines than in the wild type. Our study’s comprehensive characterization of NAC genes in <italic>C. sinensis</italic> could serve as a foundation for exploring the molecular mechanism of CsNAC-mediated drought responsiveness.</p>