AUTHOR=Baró Aina , Saldarelli Pasquale , Saponari Maria , Montesinos Emilio , Montesinos Laura 

TITLE=Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1061463

DOI=10.3389/fpls.2022.1061463

ISSN=1664-462X

ABSTRACT=<p>Transient expression of genes encoding peptides BP134 and BP178 by means of a <italic>Potato virus X</italic> (PVX) based-vector system, and treatment with synthetic peptides by endotherapy, were evaluated in the control of <italic>Xylella fastidiosa</italic> infections, in the model plant <italic>Nicotiana benthamiana</italic>. Transient production of BP178 significantly decreased disease severity compared to PVX and non-treated control (NTC) plants, without adverse effects. Plants treated with synthetic BP134 and BP178 showed consistently lower levels of disease than NTC plants. However, the coinfection with PVX-BP134 and <italic>X. fastidiosa</italic> caused detrimental effects resulting in plant death. The levels of <italic>X. fastidiosa</italic> in three zones sampled, upwards and downwards of the inoculation/treatment point, significantly decreased compared to the NTC plants, after the treatment with BP178, but not when BP178 was produced transiently. The effect of treatment and transient production of BP178 in the induction of defense-related genes was also studied. Synthetic BP178 applied by endotherapy induced the expression of <italic>ERF1</italic>, <italic>PR1a</italic>, <italic>PAL</italic>, <italic>PALII</italic> and <italic>WRKY25</italic>, while the transient expression of BP178 overexpressed the <italic>Cath, Cyc, PR4a, 9-LOX</italic> and <italic>Endochitinase B</italic> genes. Both treatments upregulated the expression of <italic>PR1</italic>, <italic>PR3</italic>, <italic>PR4</italic> and <italic>CycT9299</italic> genes compared to the NTC or PVX plants. It was concluded that the effect of BP178, either by endotherapy or by transient expression, on the control of the <italic>X. fastidiosa</italic> infections in <italic>N. benthamiana</italic>, was due in part to the induction of the plant defense system in addition to its bactericidal activity reported in previous studies. However, the protection observed when BP178 was transiently produced seems mainly mediated by the induction of plant defense, because the levels of <italic>X. fastidiosa</italic> were not significantly affected.</p>