AUTHOR=Lewis Connor D. , Preston Jill C. , Tierney Mary L. 

TITLE=CCDC22 and CCDC93, two potential retriever-interacting proteins, are required for root and root hair growth in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1051503

DOI=10.3389/fpls.2022.1051503

ISSN=1664-462X

ABSTRACT=<p>Endomembrane trafficking is essential for plant growth and often depends on a balance between secretory and endocytic pathways. VPS26C is a component of the retriever complex which has been shown to function in the recycling of integral plasma membrane proteins in human cell culture and is part of a core retriever complex in Arabidopsis that is required for root hair growth. In this work, we report a characterization of the Arabidopsis homologues of CCDC22 and CCDC93, two additional proteins required for retriever function in humans. Phylogenetic analysis indicates that <italic>CCDC22</italic> (AT1G55830) and <italic>CCDC93</italic> (AT4G32560) are single copy genes in plants that are present across the angiosperms, but like <italic>VPS26C</italic>, are absent from the grasses. Both <italic>CCDC22</italic> and <italic>CCDC93</italic> are required for root and root hair growth in Arabidopsis and localize primarily to the cytoplasm in root epidermal cells. Previous work has demonstrated a genetic interaction between VPS26C function and a VTI13-dependent trafficking pathway to the vacuole. To further test this model, we characterized the <italic>vti13 ccdc93</italic> double mutant and show that like <italic>vps26c</italic>, <italic>ccdc93</italic> is a suppressor of the <italic>vti13</italic> root hair phenotype. Together this work identifies two new proteins essential for root and root hair growth in plants and demonstrate that the endosomal pathway(s) in which CCDC93 functions is genetically linked to a VTI13-dependent trafficking pathway to the vacuole.</p>