AUTHOR=Boufleur Thaís R. , Massola Júnior Nelson S. , Becerra Sioly , Baraldi Elena , Bibiano Líllian B. J. , Sukno Serenella A. , Thon Michael R. , Baroncelli Riccardo 

TITLE=Comparative transcriptomic provides novel insights into the soybean response to Colletotrichum truncatum infection

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1046418

DOI=10.3389/fpls.2022.1046418

ISSN=1664-462X

ABSTRACT=<sec><title>Introduction</title><p>Soybean (<italic>Glycine max</italic>) is among the most important crops in the world, and its production can be threatened by biotic diseases, such as anthracnose. Soybean anthracnose is a seed-borne disease mainly caused by the hemibiotrophic fungus <italic>Colletotrichum truncatum</italic>. Typical symptoms are pre- and post-emergence damping off and necrotic lesions on cotyledons, petioles, leaves, and pods. Anthracnose symptoms can appear early in the field, causing major losses to soybean production.</p></sec><sec><title>Material and Methods</title><p>In preliminary experiments, we observed that the same soybean cultivar can have a range of susceptibility towards different strains of <italic>C. truncatum</italic>, while the same <italic>C. truncatum</italic> strain can cause varying levels of disease severity in different soybean cultivars. To gain a better understanding of the molecular mechanisms regulating the early response of different soybean cultivars to different <italic>C. truncatum</italic> strains, we performed pathogenicity assays to select two soybean cultivars with significantly different susceptibility to two different <italic>C. truncatum</italic> strains and analyzed their transcriptome profiles at different time points of interaction (0, 12, 48, and 120 h post-inoculation, hpi).</p></sec><sec><title>Results and Discussion</title><p>The pathogenicity assays showed that the soybean cultivar <italic>Gm</italic>1 is more resistant to <italic>C. truncatum</italic> strain 1080, and it is highly susceptible to strain 1059, while cultivar <italic>Gm</italic>2 shows the opposite behavior. However, if only trivial anthracnose symptoms appeared in the more resistant phenotype (MRP; <italic>Gm</italic>1-1080; <italic>Gm</italic>2-1059) upon 120 hpi, in the more susceptible phenotype (MSP; <italic>Gm</italic>-1059; <italic>Gm</italic>2- 1080) plants show mild symptoms already at 72 hpi, after which the disease evolved rapidly to severe necrosis and plant death. Interestingly, several genes related to different cellular responses of the plant immune system (pathogen recognition, signaling events, transcriptional reprogramming, and defense-related genes) were commonly modulated at the same time points only in both MRP. The list of differentially expressed genes (DEGs) specific to the more resistant combinations and related to different cellular responses of the plant immune system may shed light on the important host defense pathways against soybean anthracnose.</p></sec>