AUTHOR=Han Na , Geng Wen-ji , Li Jian , Liu Shu-ting , Zhang Jie , Wen Yi-jie , Xu Huai-hua , Li Meng-yuan , Li Yan-ru , Han Pei-pei 

TITLE=Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1040596

DOI=10.3389/fpls.2022.1040596

ISSN=1664-462X

ABSTRACT=<p>Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among <italic>T. mairei</italic> cells elicited by Ce<sup>3+</sup>, Ce<sup>4+</sup> and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce<sup>3+</sup> and 1 mM Ce<sup>4+</sup>. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out.</p>