AUTHOR=Lu Yuanda , Zhong Qiaofang , Xiao Suqin , Wang Bo , Ke Xue , Zhang Yun , Yin Fuyou , Zhang Dunyu , Jiang Cong , Liu Li , Li Jinlu , Yu Tengqiong , Wang Lingxian , Cheng Zaiquan , Chen Ling 

TITLE=A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1037901

DOI=10.3389/fpls.2022.1037901

ISSN=1664-462X

ABSTRACT=<p>Bacterial blight (BB) induced by <italic>Xanthomonas oryzae</italic> pv. <italic>oryzae</italic> (Xoo) is a devastating bacterial disease in rice. The use of disease resistance (<italic>R</italic>) genes is the most efficient method to control BB. Members of the nucleotide-binding domain and leucine-rich repeat containing protein (NLR) family have significant roles in plant defense. In this study, <italic>Xa47</italic>, a new bacterial blight <italic>R</italic> gene encoding a typical NLR, was isolated from G252 rice material, and XA47 was localized in the nucleus and cytoplasm. Among 180 rice materials tested, <italic>Xa47</italic> was discovered in certain BB-resistant materials. Compared with the wild-type G252, the knockout mutants of <italic>Xa47</italic> was more susceptible to Xoo. By contrast, overexpression of <italic>Xa47</italic> in the susceptible rice material JG30 increased BB resistance. The findings indicate that <italic>Xa47</italic> positively regulates the Xoo stress response. Consequently, <italic>Xa47</italic> may have application potential in the genetic improvement of plant disease resistance. The molecular mechanism of <italic>Xa47</italic> regulation merits additional examination.</p>