AUTHOR=Drozda Andżelika , Kurpisz Barbara , Guan Yufeng , Arasimowicz-Jelonek Magdalena , Plich Jarosław , Jagodzik Przemysław , Kuźnicki Daniel , Floryszak-Wieczorek Jolanta 

TITLE=Insights into the expression of DNA (de)methylation genes responsive to nitric oxide signaling in potato resistance to late blight disease

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1033699

DOI=10.3389/fpls.2022.1033699

ISSN=1664-462X

ABSTRACT=<p>Our previous study concerning the pathogen-induced biphasic pattern of nitric oxide (NO) burst revealed that the decline phase and a low level of NO, due to S-nitrosoglutathione reductase (GSNOR) activity, might be decisive in the upregulation of stress-sensitive genes <italic>via</italic> histone H3/H4 methylation in potato leaves inoculated with avr <italic>P</italic>. <italic>infestans</italic>. The present study refers to the NO-related impact on genes regulating DNA (de)methylation, being in dialog with histone methylation. The excessive amounts of NO after the pathogen or GSNO treatment forced the transient upregulation of histone <italic>SUVH4</italic> methylation and DNA hypermethylation. Then the diminished NO bioavailability reduced the SUVH4-mediated suppressive H3K9me2 mark on the <italic>R3a</italic> gene promoter and enhanced its transcription. However, we found that the <italic>R3a</italic> gene is likely to be controlled by the RdDM methylation pathway. The data revealed the time-dependent downregulation of the <italic>DCL3</italic>, <italic>AGO4</italic>, and <italic>miR482e</italic> genes, exerting upregulation of the targeted <italic>R3a</italic> gene correlated with <italic>ROS1</italic> overexpression. Based on these results, we postulate that the biphasic waves of NO burst in response to the pathogen appear crucial in establishing potato resistance to late blight through the RdDM pathway controlling <italic>R</italic> gene expression.</p>