AUTHOR=Um Tae Young , Hong So Yeon , Han Ji Sung , Jung Ki Hong , Moon Sunok , Choi Beom-Soon , Basnet Prakash , Chung Young Soo , Lee Seon Woo , Yang Won Tae , Kim Doh Hoon 

TITLE=Gibberellic acid sensitive dwarf encodes an ARPC2 subunit that mediates gibberellic acid biosynthesis, effects to grain yield in rice

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1027688

DOI=10.3389/fpls.2022.1027688

ISSN=1664-462X

ABSTRACT=<p>The plant hormone gibberellic acid (GA) is important for plant growth and productivity. Actin-related proteins (ARPs) also play central roles in plant growth, including cell elongation and development. However, the relationships between ARPs and GA signaling and biosynthesis are not fully understood. Here, we isolated <italic>OsGASD</italic>, encoding an ARP subunit from rice (<italic>Oryza sativa</italic>), using the Ac/Ds knockout system. The <italic>osgasd</italic> knockout (Ko) mutation reduced GA3 content in shoots as well as plant growth and height. However, GA application restored the plant height of the <italic>osgasd</italic> Ko mutant to a height similar to that of the wild type (WT). Rice plants overexpressing <italic>OsGASD</italic> (Ox) showed increased plant height and grain yield compared to the WT. Transcriptome analysis of flag leaves of <italic>OsGASD</italic> Ox and <italic>osgasd</italic> Ko plants revealed that OsGASD regulates cell development and the expression of elongation-related genes. These observations suggest that <italic>OsGASD</italic> is involved in maintaining GA homeostasis to regulate plant development, thereby affecting rice growth and productivity.</p>