AUTHOR=Vladejić Jovanka , Yang Fen , Dvořák Tomaštíková Eva , Doležel Jaroslav , Palecek Jan J. , Pecinka Ales TITLE=Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis JOURNAL=Frontiers in Plant Science VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1023358 DOI=10.3389/fpls.2022.1023358 ISSN=1664-462X ABSTRACT=
The integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identification and analysis of four uncharacterized Arabidopsis BRCT5 DOMAIN CONTAINING PROTEINs (BCPs). Proteins with the BRCT5 domain are frequently involved in the maintenance of genome stability across eukaryotes. The screening for sensitivity to induced DNA damage identified BCP1 as the most interesting candidate. We show that BCP1 loss of function mutants are hypersensitive to various types of DNA damage and accumulate an increased number of dead cells in root apical meristems upon DNA damage. Analysis of publicly available