AUTHOR=Du Jie , Zhang Cuiyang , Long Qingshan , Zhang Liang , Chen Wu , Liu Qingshu 

TITLE=Characterization of a pathway−specific activator of edeine biosynthesis and improved edeine production by its overexpression in Brevibacillus brevis

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1022476

DOI=10.3389/fpls.2022.1022476

ISSN=1664-462X

ABSTRACT=<p>Edeines are a group of non-ribosomal antibacterial peptides produced by <italic>Brevibacillus brevis</italic>. Due to the significant antibacterial properties of edeines, increasing edeine yield is of great interest in biomedical research. Herein, we identified that EdeB, a member of the ParB protein family, significantly improved edeine production in <italic>B. brevis</italic>. First, overexpression of <italic>edeB</italic> in <italic>B. brevis</italic> X23 increased edeine production by 92.27%. Second, <italic>in vitro</italic> bacteriostasis experiment showed that <italic>edeB</italic>-deletion mutant exhibited less antibacterial activity. Third, RT-qPCR assay demonstrated that the expression of <italic>edeA</italic>, <italic>edeQ</italic>, and <italic>edeK</italic>, which are key components of the edeine biosynthesis pathway, in <italic>edeB</italic>-deletion mutant X23(ΔedeB) was significantly lower than that in wild-type <italic>B. brevis</italic> strain X23. Finally, electrophoretic mobility shift assay (EMSA) showed that EdeB directly bound to the promoter region of the edeine biosynthetic gene cluster (<italic>ede</italic> BGC), suggesting that EdeB improves edeine production through interaction with <italic>ede</italic> BGC in <italic>B. brevis</italic>.</p>