AUTHOR=Zhao Wei , Guo Lirong , Yang Yu , Wang Yan , Yang Li , Wei Cuimei , Guo Jian , Yan Kan , Chen Haijuan , Yang Zhigang , Li Yimeng 

TITLE=Complete chloroplast genome sequences of Phlomis fruticosa and Phlomoides strigosa and comparative analysis of the genus Phlomis sensu lato (Lamiaceae)

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1022273

DOI=10.3389/fpls.2022.1022273

ISSN=1664-462X

ABSTRACT=<p>The taxonomic terms “<italic>Phlomis</italic>” and “<italic>Phlomoides</italic>” had been used to describe two sections within the genus <italic>Phlomis</italic> belonging to the Lamiaceae family. Recently, phylogenetic analyses using molecular markers showed that <italic>Phlomis</italic> and <italic>Phlomoides</italic> formed two monophyletic clades, and thus they are generally accepted as separate genera. In this study, we assembled the complete chloroplast genome of <italic>Phlomis fruticosa</italic>, which is the first reported chloroplast genome belonging to <italic>Phlomis</italic> genus, as well as the complete chloroplast genome of <italic>Phlomoides strigosa</italic> belonging to <italic>Phlomoides</italic> genus. The results showed that the length of chloroplast genome was 151,639 bp (<italic>Phlomis fruticosa</italic>) and 152,432 bp (<italic>Phlomoides strigosa</italic>), with conserved large single copy regions, small single copy regions, and inverted repeat regions. 121 genes in <italic>Phlomis fruticosa</italic> and 120 genes in <italic>Phlomoides strigosa</italic> were annotated. The chloroplast genomes of <italic>Phlomis fruticosa</italic>, <italic>Phlomoides strigosa</italic>, and three reported <italic>Phlomoides</italic> species, as well as those of 51 species from the Lamiaceae family, which covered 12 subfamilies, were subjected to phylogenetic analyses. The <italic>Phlomis</italic> and <italic>Phlomoides</italic> species were split into two groups, which were well supported by both maximum likelihood and Bayesian inference tree analyses. Our study provided further evidence to recognize <italic>Phlomis</italic> and <italic>Phlomoides</italic> as independent genera.</p>