AUTHOR=Yang Fang , Lv Guanghui 

TITLE=Combined analysis of transcriptome and metabolome reveals the molecular mechanism and candidate genes of Haloxylon drought tolerance

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1020367

DOI=10.3389/fpls.2022.1020367

ISSN=1664-462X

ABSTRACT=<p><italic>Haloxylon ammodendron</italic> and <italic>Haloxylon persicum</italic>, as typical desert plants, show strong drought tolerance and environmental adaptability. They are ideal model plants for studying the molecular mechanisms of drought tolerance. Transcriptomic and metabolomic analyses were performed to reveal the response mechanisms of <italic>H. ammodendron</italic>
 and <italic>H. persicum</italic>
 to a drought environment at the levels of transcription and physiological metabolism. The results showed that the morphological structures of <italic>H. ammodendron</italic>
 and <italic>H. persicum</italic>
 showed adaptability to drought stress. Under drought conditions, the peroxidase activity, abscisic acid content, auxin content, and gibberellin content of <italic>H. ammodendron</italic> increased, while the contents of proline and malondialdehyde decreased. The amino acid content of <italic>H. persicum</italic> was increased, while the contents of proline, malondialdehyde, auxin, and gibberellin were decreased. Under drought conditions, 12,233 and 17,953 differentially expressed genes (DEGs) were identified in <italic>H. ammodendron</italic>
 and <italic>H. persicum</italic>
, respectively, including members of multiple transcription factor families such as FAR1, AP2/ERF, C2H2, bHLH, MYB, C2C2, and WRKY that were significantly up-regulated under drought stress. In the positive ion mode, 296 and 452 differential metabolites (DEMs) were identified in <italic>H. ammodendron</italic>
 and <italic>H. persicum</italic>, respectively; in the negative ion mode, 252 and 354 DEMs were identified, primarily in carbohydrate and lipid metabolism. A combined transcriptome and metabolome analysis showed that drought stress promoted the glycolysis/gluconeogenesis pathways of <italic>H. ammodendron</italic>
 and <italic>H. persicum</italic>
 and increased the expression of amino acid synthesis pathways, consistent with the physiological results. In addition, transcriptome and metabolome were jointly used to analyze the expression changes of the genes/metabolites of <italic>H. ammodendron</italic>
 and <italic>H. persicum</italic>
 that were associated with drought tolerance but were regulated differently in the two plants. This study identified drought-tolerance genes and metabolites in <italic>H. ammodendron</italic>
 and <italic>H. persicum</italic>
 and has provided new ideas for studying the drought stress response of <italic>Haloxylon</italic>.</p>