AUTHOR=Ullah Abd , Zeng Fanjiang , Tariq Akash , Asghar Muhammad Ahsan , Saleem Khansa , Raza Ali , Naseer Muhammad Asad , Zhang Zhihao , Noor Javaria TITLE=Exogenous naphthaleneacetic acid alleviated alkalinity-induced morpho-physio-biochemical damages in Cyperus esculentus L. var. sativus Boeck JOURNAL=Frontiers in Plant Science VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1018787 DOI=10.3389/fpls.2022.1018787 ISSN=1664-462X ABSTRACT=

Cyperus esculentus L. var. sativus Boeck (commonly called Chufa) is a perennial species that produces nutritious underground tubers and contributes to the diet and health of human worldwide. However, it is salt-sensitive and its adaptation to salinity stress remains an enigma. Naphthaleneacetic acid (NAA) plays a vital role in regulating plant salt stress tolerance. Thus, we aimed to investigate the impact of NAA (150 mg/L) application on growth and physio-biochemical response mechanisms of Chufa plants to different levels of salinity stress (0-, 90-, and 180 mM of alkaline stress ([1:1 ratio of Na₂CO₃ and NaHCO₃]). In response to increasing stress levels, shoot-root growth decreased, whereas malondialdehyde (MDA), hydrogen peroxide (H₂O₂), osmolytes (soluble protein, proline, and soluble sugars), and activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) significantly increased. Alkalinity led to significant increase in Na⁺ and Cl^–, but decrease in Mg²⁺ concentration in both roots and leaves; however, K⁺ decreased significantly in leaves under both stresses. Additionally, NO3−and. levels, nitrate reductase (NR) activities, and glutamate synthase (GOGAT) decreased significantly. However, glutamine synthetase (GS) increased non-significantly at 90 mM but declined at 180 mM. Foliar NAA application reduced Na⁺ and Cl^-, MDA, and H₂O₂ but increased photosynthetic pigments, K⁺ and Mg²⁺, osmolytes, nitrogen (N) metabolism, and upregulating the enzymatic antioxidant system to reduce oxidative stress under alkaline conditions. Hence, our findings manifest that NAA application is an effective strategy that can be utilized to enhance tolerance of chufa plants to alkaline stress. Future studies should explore whether NAA can positively alter the nutrient composition of chufa tubers at deeper molecular levels, which might offer solutions to nutritious problems in developing countries.