AUTHOR=Song Huifang , Fu Xinxuan , Li Juan , Niu Tianzeng , Shen Jie , Wang Xi , Li Yunling , Hou Qinwen , Liu Ake 

TITLE=Phylogenetic analysis and expression profiles of jasmonate ZIM-domain gene family provide insight into abiotic stress resistance in sunflower

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1010404

DOI=10.3389/fpls.2022.1010404

ISSN=1664-462X

ABSTRACT=<p>Jasmonate ZIM-domain (JAZ) proteins act as inhibitory factors of the jasmonic acid (JA) pathway, which is involved in regulating plant development and defense responses. However, there are no extensive studies available on <italic>JAZ</italic> genes in sunflower (<italic>Helianthus annuus</italic> L.). In this study, the phylogenetic analysis of 139 putative <italic>JAZ</italic> genes from eight plants demonstrated that these <italic>JAZs</italic> could be divided into five groups (Groups I–V), and the 27 sunflower <italic>JAZs</italic> (<italic>HaJAZs</italic>) were classified into these five groups. All groups contained genes from both monocotyledons and dicotyledons, indicating that the emergence of <italic>JAZ</italic> genes predates the differentiation of monocotyledons and dicotyledons. Both segmental and tandem duplications contributed greatly to this gene family’s expansion in sunflower, especially in Group II. Moreover, the expression profiles of <italic>HaJAZ</italic> genes under normal conditions, hormone treatments or abiotic stresses were analyzed based on RNA-seq data. <italic>HaJAZ2</italic> may be undergoing pseudogenization as a nonfunctional gene because it was not expressed in any tissue. Many <italic>HaJAZ</italic> genes in roots upregulated their expression when involved in responding to exogenous hormones, especially methyl-jasmonate. The abiotic stress treatments of sunflower showed that <italic>HaJAZ5</italic>, <italic>HaJAZ15</italic>, <italic>HaJAZ17</italic>, <italic>HaJAZ20</italic>, and <italic>HaJAZ21</italic> tend to be sensitive to certain abiotic stresses. <italic>HaJAZs</italic> from different groups may share similar functions but also exercise their unique functions when responding to abiotic stresses. We speculated that this gene family was conserved in sequence but varied in its expression among duplicated <italic>HaJAZ</italic> genes, which implies that they may confer neofunctionalization in the adaptation to abiotic stresses; this work provides insight into the resistance of sunflowers and their adaptation to diverse environmental conditions.</p>